KIR3.4 polyclonal antibody

Product Specifications

Background

G protein-coupled inwardly rectifying potassium channels (Kir3.1 through Kir3.4) are coupled to numerous neurotransmitter receptors in the brain and are abundantly expressed in the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex and thalamus regions of the brain. Also known as GIRK, Kir3 potassium channels localize to the soma and dendrites as well as axons of neurons. Liberated Gbg subunits from G protein heterotrimers bind to and regulate Kir3 channel activity. Gb3- and Gb4-containing Gbg dimers bind directly to cytoplasmic domains of Kir3 proteins and increase the K+ current while Gb5-containing Gbg dimers inhibit Kir3 K+ current. Kir3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor activates receptor tyrosine kinase B, which then phosphorylates Kir3 tyrosine residues, effectively inactivating the Kir3 channels.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

G protein-activated inward rectifier potassium channel 4; GIRK-4; GIRK4; Cardiac inward rectifier; CIR; Heart KATP channel; Inward rectifier K (+) channel Kir3.4; IRK-4; KATP-1; IRK4; KATP1; Potassium channel, inwardly rectifying subfamily J member 5; KCNJ5

Swiss Prot

P48544

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the 374-419 of Human KIR3.4.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .