Frizzled-6 (G169) polyclonal antibody

Product Specifications

Background

Frizzled-6 (FZD6, also known as frizzled homolog 6 in Drosophila) is a seven-transmembrane domain receptor that binds the Wnt ligand and influences macroscopic hair patterning and other tissue polarity events. Frizzled-6 protein contains a signal peptide and a cysteine-rich domain (CRD) in the N-terminal extracellular region, and does not contain a C-terminal PDZ domain-binding motif. Frizzled-3 and frizzled-6 influence neural tube closure and the planar orientation of hair bundles on a subset of auditory and vestibular sensory cells. Madin-Darby canine kidney (MDCK) cells are competent to form tubules in vitro and express the frizzled-6 receptor, which is known to form a complex with Wnt-4 through the CRD in this cell type. Frizzled-6 is expressed as a 4.4 kb mRNA in various human tissues, including adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Frizzled6; Frizzled 6; Fz-6; hFz6; FZD6; FZD-6

Swiss Prot

O60353

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 169-214 of Human Frizzled-6.

Conjugation

Unconjugated

Related Pathways

Wntβ catenin signaling

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 79 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Frizzled-6 (G169) polyclonal antibody detects endogenous levels of Frizzled-6 protein.

Applications Notes

Western blot (WB) analysis of Frizzled-6 (G169) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate