FPR1 (I203) polyclonal antibody

Product Specifications

Background

The N-formyl peptide receptor (FPR) is a chemotactic G protein-coupled receptor (GPCR) that is found on the surface of phagocytic leukocytes, such as neutrophils and monocytes. The human FPR family comprises three members, FPR, FPRL1 (also designated lipoxin A4 receptor) and FPRL2, and each family member contains specific residues, which are responsible for determining its ligand specificity. FPR, a seven transmembrane-domain receptor, primarily binds the chemoattractant N-formyl-methionyl-leucyl-phenylalanine (fMLP), which activates several biological processes, including chemotaxis, transcriptional activation, and actin reorganization. FPR also mediates the inhibition of neutrophil migration through binding to specific peptide fragments of annexin I, which causes calcium transients and affects inflammatory responses.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

FMet-Leu-Phe receptor; fMLP receptor; N-formyl peptide receptor; Short name=FPR; N-formylpeptide chemoattractant receptor

Swiss Prot

P21462

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the 203-248 of Human FPR1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

FPR1 (I203) polyclonal antibody detects endogenous levels of FPR1 protein.

Applications Notes

Western blot (WB) analysis of FPR1 (I203) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat brain tissue lysate