KALRN polyclonal antibody

Product Specifications

Background

HAP1 (huntingtin-associated protein 1) binds to huntingtin. Huntingtin is a protein that contains a polyglutamine region and when the number of glutamine repeats exceeds 35, the gene encodes a version of huntingtin that leads to Huntington’s disease (HD) . The ability of HAP1 to bind to huntingtin is enhanced by an expanded polyglutamine repeat region. HAP1 shows neuronal localization and moves with huntingtin in nerve fibers. HAP1 is primarily expressed in brain tissue, with greater expression in the olfactory bulb and brain stem. Mouse HAP1 is localized to membrane-bound organelles including large endosomes, tubulovesicular structures and budding vesicles in neurons. Duo, also designated huntingtin-associated protein interacting protein or HAPIP, binds Huntingtin-associated protein 1 (HAP1) and may have a role in vesicle trafficking and cytoskeletal function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Kalirin; Huntingtin-associated protein-interacting protein; Protein Duo; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain; DUET; DUO; HAPIP; TRAD

Swiss Prot

O60229

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 895-940 of Human KALRN.

Conjugation

Unconjugated

Applications

WB ICC

Dilution

WB: 1:500~1:1000 ICC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 192, 350 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

KALRN polyclonal antibody detects endogenous levels of KALRN protein.

Applications Notes

Western blot (WB) analysis of GPR15 pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:The Uterus tissue lysate of Mouse (40ug) Lane5:The Uterus tissue lysate of Rat (40ug)