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CA5A polyclonal antibody

Product Specifications

Background

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA5A gene encodes for the CA V protein which is expressed in the mitochondrial matrix in liver tissues, but can also be detected in many other tissues. In ureagenesis, the CA V protein may be involved in providing bicarbonate to carbamoyl phosphate synthetase.

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Carbonic anhydrase 5A, mitochondrial; Carbonate dehydratase VA; Carbonic anhydrase VA; CA-VA; CA5A; CA5

Swiss Prot

P35218

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 223-268 of Human CA5A.

Conjugation

Unconjugated

Applications

WB ICC

Dilution

WB: 1:500~1:1000 ICC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CA5A polyclonal antibody detects endogenous levels of CA5A protein.

Applications Notes

Western blot (WB) analysis of CA5A polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate

Host or Source

Rabbit

CAS Number

9007-83-4

Available Sizes

Curated Selection

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