ARHGEF10 polyclonal antibody
Product Specifications
Background
ARHGEF10 (rho guanine nucleotide exchange factor 10), also known as GEF10 or KIAA0294, is a 1,369 amino acid protein that contains one DBL-homology domain and is thought to play a role in myelination of peripheral nerves, specifically during development. ARHGEF10 is expressed as four alternatively spliced isoforms that are present at low levels in ovary, lung, testis and kidney, with considerably higher expression in the central and peripheral nervous systems. Defects in the gene encoding ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV), an autosomal dominant disorders that is characterized by a reduction in nerve velocities resulting from lack of proper myelination.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Rho guanine nucleotide exchange factor 10; ARHGEF10; KIAA0294
Swiss Prot
O15013
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human ARHGEF10.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 152 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ARHGEF10 polyclonal antibody detects endogenous levels of ARHGEF10 protein.
Applications Notes
Western blot (WB) analysis of ARHGEF10 (R370) pAb at 1:1000 dilution Lane1:C6 whole cell lysate (40ug) Lane2:3T3-L1 whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:A375 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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