Cdk1/Cdc2 (phospho-Y15) polyclonal antibody

Product Specifications

Background

Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2; CDC28A; CDKN1; P34CDC2

Swiss Prot

P06493

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Cdk1/Cdc2 around the phosphorylation site of Serine 795.

Conjugation

Unconjugated

Related Pathways

Cell Cycle G2 M DNA Damage Signaling Pathway

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 34 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Cdk1/Cdc2 (Y15) polyclonal antibody detects endogenous levels of Cdk1/Cdc2 protein only when phosphorylated at Tyr15.

Applications Notes

Western blot (WB) analysis of Cdc2 (phospho-Y15) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:Hela treated with UV (10min) whole cell lysate (40ug) Lane3:Hela treated with UV (20min) whole cell lysate (40ug) Lane4:Hela treated with UV (30min) whole cell lysate (40ug)