4E-BP1 (phospho-S65) polyclonal antibody
Product Specifications
Background
The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP1; 4EBP1; PHAS I; PHASI
Swiss Prot
Q13541
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Serine 65.
Conjugation
Unconjugated
Related Pathways
PI3K AKT signaling, Hypoxia Signaling, Insulin &Glucose Signaling, MTOR signaling
Applications
IHC
Dilution
IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 15 to 20 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-4E-BP1 (S65) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Ser65.
Host or Source
Rabbit
Available Sizes
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