Synapsin I (phospho-S62) polyclonal antibody

Product Specifications

Background

Synapsin I is a member of the synapsin family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations of the Synapsin I gene may be associated with X linked disorders with primary neuronal degeneration such as Rett syndrome.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Synapsin-1; Synapsin 1; Synapsin1; Brain protein 4.1; Synapsin I; SYN1

Swiss Prot

P17600

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Synapsin I around the phosphorylation site of Serine 62.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Synapsin I (S62) polyclonal antibody detects endogenous levels of Synapsin I protein when phosphorylated at Ser62.

Applications Notes

Western blot (WB) analysis of p-SYN1 (S62) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with PMA (100nM,30min) Lane2:Raw264.7 cell lysate treated with PMA (00nM,30min) Lane3:H9C2 cell lysate treated with PMA (100nM,30min)