Rad52 (phospho-Y104) polyclonal antibody
Product Specifications
Background
DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DNA repair protein RAD52 homolog; RAD52
Swiss Prot
P43351
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Rad52 around the phosphorylation site of Tyrosine 104.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 46 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Rad52 (Y104) polyclonal antibody detects endogenous levels of Rad52 protein when phosphorylated at Tyr104.
Applications Notes
Western blot (WB) analysis of p-Rad52 (Y104) polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Mouse spleen tissue lysate Lane3:Rat spleen tissue lysate
Host or Source
Rabbit
Available Sizes
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