PLC γ1 (phospho-Y1253) polyclonal antibody

Product Specifications

Background

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds α1β1 Integrin and modulates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C2; PLC2I; Phospholipase C-gamma-1; PLC-gamma-1; PLCG1; PLC1; PLCγ1

Swiss Prot

P19174

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human PLC γ1 around the phosphorylation site of Tyrosine 1253.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, Phospholipase Signaling, Regulation of Actin Dynamics, Tumor Angiogenesis, T cell receptor signaling

Applications

WB ICC

Dilution

WB: 1:500~1:1000 ICC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 155 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-PLC γ1 (Y1253) polyclonal antibody detects endogenous levels of PLC γ1 protein when phosphorylated at Tyr1253.

Applications Notes

Western blot (WB) analysis of p-PLC γ1 (Y1253) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:Mouse liver tissue lysate Lane3:H9C2 cell lysate treated with EGF (0.1ng/ML,30mins)