PFKFB2 (phospho-S483) polyclonal antibody
Product Specifications
Background
PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
PFK-2 car; PFK-2 cardiac; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase; PFKFB2
Swiss Prot
O60825
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human PFKFB2 around the phosphorylation site of Serine 483.
Conjugation
Unconjugated
Related Pathways
Warburg Effect, Glutamine Metabolism
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 58 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-PFKFB2 (S483) polyclonal antibody detects endogenous levels of PFKFB2 protein only when phosphorylated at Ser483.
Applications Notes
Western blot (WB) analysis of p-PFKFB2 (S483) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with H2O2 (100µM,30mins) Lane2:sp2/0 cell lysate treated with H2O2 (100µM,30mins) Lane3:H9C2 cell lysate treated with H2O2 (100µM,30mins)
Host or Source
Rabbit
Available Sizes
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