PAK3 (phospho-S154) polyclonal antibody
Product Specifications
Background
Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3; PAK3; OPHN3; PAKβ
Swiss Prot
O75914
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human PAK3 around the phosphorylation site of Serine 154.
Conjugation
Unconjugated
Related Pathways
Regulation of Microtubule Dynamics
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 65 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-PAK3 (S154) polyclonal antibody detects endogenous levels of PAK3 protein only when phosphorylated at Ser154.
Applications Notes
Western blot (WB) analysis of p-PAK3 (S154) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:sp2/0 cell lysate treated with EGF (0.1ng/ML,30mins) Lane3:H9C2 cell lysate treated with EGF (0.1ng/ML,30mins)
Host or Source
Rabbit
Available Sizes
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