P27 Kip1 (phospho-T198) polyclonal antibody

Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits, designated Cdks, and activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been recently described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner, p16 and a more recently described p16 related inhibitor designated p15. A p21 related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGFβ-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and to a lesser extent with cyclin E and Cdk2.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-p27 Kip1 (T198) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ML,30mins) Lane3:H9C2 cell lysate treated with EGF (0.1ng/ML,30mins)