P130 Cas (phospho-Y249) polyclonal antibody

Product Specifications

Background

The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH) -3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas; BCAR1; CAS; CASS1; CRKAS

Swiss Prot

P56945

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human p130 Cas around the phosphorylation site of Tyrosine 249.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, ErbB HER Signaling

Applications

WB IHC ICC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 130 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-p130 Cas (Y410) polyclonal antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Tyr410.

Applications Notes

Western blot (WB) analysis of p-p130 Cas (Y249) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ML,30mins) Lane3:PC12 cell lysate treated with EGF (0.1ng/ML,30mins)