MSK1 (phospho-T581) polyclonal antibody
Product Specifications
Background
MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) . Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1
Swiss Prot
O75582
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Threonine 581.
Conjugation
Unconjugated
Related Pathways
Signaling Pathways Activating p38 MAP Kinase, Epigenetic Writers and Erasers of Histone H3, Epigenetic Writers and Erasers of Histones H2A H2B and H4
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-MSK1 (T581) polyclonal antibody detects endogenous levels of MSK1 protein when phosphorylated at Thr581.
Applications Notes
Western blot (WB) analysis of p-MSK1 (T581) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate teated with UV (UV,24h) Lane2:sp2/0 cell lysate treated with UV (UV,24h) Lane3:Rat liver tissue lysate
Host or Source
Rabbit
Available Sizes
Curated Selection
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