MEK1 (phospho-T286) polyclonal antibody

Product Specifications

Background

MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1

Swiss Prot

Q02750

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Threonine 286.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Warburg Effect, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, MAPK Erk in Growth and Differentiation Pathway

Applications

WB IHC

Dilution

WB:1:500~1:1000 IHC:1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 43 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-MEK1 (T286) polyclonal antibody detects endogenous levels of MEK1 protein only when phosphorylated at Thr286.

Applications Notes

Western blot (WB) analysis of p-MEK1 (T286) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with PMA (100nM,30mins) Lane2:NIH-3T3 cell lysate treated with UV (24h) Lane3:PC12 cell lysate treated with PMA (100nM,15mins)