LEF-1 (phospho-S42) polyclonal antibody
Product Specifications
Background
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
LEF-1; TCF10; TCF1ALPHA; TCF7L3
Swiss Prot
Q9UJU2
Reactivity
Human, Rat
Immunogen
Synthetic phosphopeptide derived from human LEF-1 around the phosphorylation site of Serine 42
Conjugation
Unconjugated
Related Pathways
Wntβ catenin signaling, Contribution of ECM and Cytoskeletal Factors to EMT
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 53 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-LEF-1 (S42) pAb detects endogenous levels of LEF-1 protein only when phosphorylated at Ser42.
Applications Notes
Western blot (WB) analysis of p-LEF-1 (S42) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40µg) Lane2:LOVO whole cell lysate (40µg) Lane3:DLD whole cell lysate (40µg) Lane4:The Thymus lysate of Rat (40µg)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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