LEF-1 (phospho-S42) polyclonal antibody

Product Specifications

Background

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

LEF-1; TCF10; TCF1ALPHA; TCF7L3

Swiss Prot

Q9UJU2

Reactivity

Human, Rat

Immunogen

Synthetic phosphopeptide derived from human LEF-1 around the phosphorylation site of Serine 42

Conjugation

Unconjugated

Related Pathways

Wntβ catenin signaling, Contribution of ECM and Cytoskeletal Factors to EMT

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 53 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-LEF-1 (S42) pAb detects endogenous levels of LEF-1 protein only when phosphorylated at Ser42.

Applications Notes

Western blot (WB) analysis of p-LEF-1 (S42) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40µg) Lane2:LOVO whole cell lysate (40µg) Lane3:DLD whole cell lysate (40µg) Lane4:The Thymus lysate of Rat (40µg)