NF2/Merlin (phospho-S518) polyclonal antibody
Product Specifications
Background
Neurofibromin 2 (NF2) is a tumor suppressor gene encoding the protein Merlin. Merlin is closely related to the ERM (ezrin, radixin, moesin) family of proteins. The precise funtion of Merlin is not clear. It is thought to provide a link between the actin cytoskeleten and membrane associated proteins, playing a role in transduction of extracellular signals. It has been implicated in cell proliferation and cellular motility. Mutations in the NF2 gene cause neurofibromatosis type II, a condition characterised by the development of tumors in the central nervous system.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin; NF2; SCH
Swiss Prot
P35240
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Merlin around the phosphorylation site of Serine 518.
Conjugation
Unconjugated
Related Pathways
Hippo Signaling
Applications
WB IF
Dilution
WB: 1:500~1:1000 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 70 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-NF2/Merlin (S518) polyclonal antibody detects endogenous levels of NF2/Merlin protein only when phosphorylated at Ser518
Applications Notes
Western blot (WB) analysis of p-NF2 (S518) pAb at 1:500 dilution Lane1:A375 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:The Uterus tissue lysate of Rat (40ug) Lane4:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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