GAP43 (phospho-S41) polyclonal antibody
Product Specifications
Background
GAP-43 (growth associated protein 43, B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1 is a crucial component for regenerative response in the nervous system. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The neuron-specific Elav/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons and leads to increased levels of GAP-43 protein.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46; GAP43
Swiss Prot
P17677
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human GAP43 around the phosphorylation site of Serine 41.
Conjugation
Unconjugated
Related Pathways
Neuronal and Glial Cell Marker Atlas
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 25, 48 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-GAP-43 (S41) polyclonal antibody detects endogenous levels of GAP-43 protein only when phosphorylated at Ser41.
Applications Notes
Western blot (WB) analysis of p-GAP-43 (S41) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with PMA (100nM,30mins) Lane2:Raw264.7 cell lysate treated with PMA (100nM,30mins) Lane3:PC12 cell lysate treated with PMA (100nM,30mins)
Host or Source
Rabbit
Available Sizes
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