CREB (phospho-S129) polyclonal antibody

Product Specifications

Background

Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1

Swiss Prot

P16220

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human CREB around the phosphorylation site of Serine 129.

Conjugation

Unconjugated

Related Pathways

Signaling Pathways Activating p38 MAP Kinase

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-CREB (S129) polyclonal antibody detects endogenous levels of CREB-1 protein only when phosphorylated at Ser129.

Applications Notes

Western blot (WB) analysis of p-CREB-1 (S129) pAb at 1:500 dilution Lane1:A375 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Brain tissue lysate of Rat (40ug) Lane4:BV2 whole cell lysate (40ug)