CPI-17 (phospho-T38) polyclonal antibody
Product Specifications
Background
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17; CPI17; PPP1INL; PPP1R14A
Swiss Prot
Q96A00
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human CPI-17 around the phosphorylation site of Threonine 38.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-CPI-17 (T38) polyclonal antibody detects endogenous levels of CPI-17 polyclonal antibody protein only when phosphorylated at Thr38.
Applications Notes
Western blot (WB) analysis of p-CPI-17 (T38) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:The Brain tissue lysate of Mouse (20ug)
Host or Source
Rabbit
Available Sizes
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