Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Cortactin (phospho-Y421) polyclonal antibody

Product Specifications

Background

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486) . In vitro, cortactin binds to and cross links F actin into meshworks.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1

Swiss Prot

Q14247

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Cortactin (Y421) polyclonal antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr421.

Applications Notes

Western blot (WB) analysis of p-Cortactin (Y421) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with H2O2 (100uM,15mins)

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

HIPPI (IFT57) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2G3]
TA500917AM 100 µL

HIPPI (IFT57) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2G3]

Sign In for Pricing
View Details
EnzyChrom™ Superoxide Dismutase Assay Kit
ESOD-100 100 Tests

EnzyChrom™ Superoxide Dismutase Assay Kit

Sign In for Pricing
View Details
SA 4503 Dihydrochloride
TRC-S139590-50MG 50 mg

SA 4503 Dihydrochloride

Sign In for Pricing
View Details
C16orf57 (USB1) Mouse Monoclonal Antibody [Clone ID: OTI4B1]
CF811338 100 µg

C16orf57 (USB1) Mouse Monoclonal Antibody [Clone ID: OTI4B1]

Sign In for Pricing
View Details
Human TTC34 activation kit by CRISPRa
GA119224 1 Kit

Human TTC34 activation kit by CRISPRa

Sign In for Pricing
View Details
ALDH6A1 (NM_005589) Human Over-expression Lysate
LC401715 20 µg

ALDH6A1 (NM_005589) Human Over-expression Lysate

Sign In for Pricing
View Details