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Cortactin (phospho-Y421) polyclonal antibody

Product Specifications

Background

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486) . In vitro, cortactin binds to and cross links F actin into meshworks.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1

Swiss Prot

Q14247

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Contribution of ECM and Cytoskeletal Factors to EMT, Adherens Junction signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Cortactin (Y421) polyclonal antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr421.

Applications Notes

Western blot (WB) analysis of p-Cortactin (Y421) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with H2O2 (100uM,15mins)

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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