Connexin 43 (phospho-S367) polyclonal antibody

Product Specifications

Background

Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser367 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Gap junction alpha-1 protein; Connexin-43; Connexin 43; Connexin43; Cx43; Cx-43; Gap junction 43 kDa heart protein; GJA1; GJAL

Swiss Prot

P17302

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Connexin 43 around the phosphorylation site of Serine 367.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 43 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Connexin 43 (S367) polyclonal antibody detects endogenous levels of Connexin 43 protein when phosphorylated at Ser367.

Applications Notes

Western blot (WB) analysis of p-Connexin 43 (S367) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:SGC7901 treated with PMA (50ng/ml,10 minutes) whole cell lysate (40ug) Lane3:The Heart tissue lysate of Mouse (40ug) Lane4:The Testis tissue lysate of Rat (40ug)