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Histone H2A.X (phospho-S139) polyclonal antibody

Product Specifications

Background

H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X

Swiss Prot

P16104

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Histone Lysine Methylation Pathway

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 15 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Histone H2A.X (S139) polyclonal antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.

Applications Notes

Western blot (WB) analysis of p-Histone H2A.X (S139) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with colchicine (0.2µg/ML,24h) Lane2:sp2/0 cell lysate treated with colchicine (0.2µg/ML,24h) Lane3:PC12 cell lysate treated with colchicine (0.2µg/ML,24h)

Host or Source

Rabbit

Available Sizes

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