C-Met (phospho-Y1349) polyclonal antibody
Product Specifications
Background
C-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF) . The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprised of an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET
Swiss Prot
P08581
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyrosine 1349.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 156 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-c-Met (Y1349) polyclonal antibody detects endogenous levels of c-Met protein only when phosphorylated at Tyr1349.
Applications Notes
Western blot (WB) analysis of p-Met (Y1349) polyclonal antibody at 1:500 dilution Lane1:THP-1 cell lysate treated with EGF (0.1ng/ml,30mins) Lane2:Hela cell lysate treated with EGF (0.1ng/ml,30mins)
Host or Source
Rabbit
Available Sizes
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