C-Met (phospho-Y1349) polyclonal antibody

Product Specifications

Background

C-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF) . The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprised of an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET

Swiss Prot

P08581

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyrosine 1349.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 156 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-c-Met (Y1349) polyclonal antibody detects endogenous levels of c-Met protein only when phosphorylated at Tyr1349.

Applications Notes

Western blot (WB) analysis of p-Met (Y1349) polyclonal antibody at 1:500 dilution Lane1:THP-1 cell lysate treated with EGF (0.1ng/ml,30mins) Lane2:Hela cell lysate treated with EGF (0.1ng/ml,30mins)