EIF4E (phospho-S209) polyclonal antibody

EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

EIF4E (phospho S209) ; eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; 1977; EIF4E; 133440; P06730; CBP; EIF4EL1; EIF4F; MGC111573

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-eIF4E (S209) pAb at 1:500 dilution Lane1:The Lung tissue lysate of Mouse (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:3T3-L1 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:A2780 whole cell lysate (40ug) Lane6:SK-OVCAR3 whole cell lysate (40ug) Lane7:Panc1 whole cell lysate (20ug)