VASP (phospho-S238) polyclonal antibody

Product Specifications

Background

VASP (vasodilator-stimulated phosphoprotein), is involved in the maintenance of cytoarchitecture by interacting with actin-like filaments. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in WAS patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is a protein of between 46 and 50 kDa that is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Vasodilator-stimulated phosphoprotein; VASP

Swiss Prot

P50552

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human VASP around the phosphorylation site of Serine 238.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 40, 48 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-VASP (S238) polyclonal antibody detects endogenous levels of VASP protein only when phosphorylated at Ser238.

Applications Notes

Western blot (WB) analysis of p-VASP (S238) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with forskolin (10nM, 72h) Lane2:Raw264.7 cell lysate treated with forskolin (10nM, 72h) Lane3:PC12 cell lysate treated with forskolin (10nM, 72h)