AKT2 (phospho-S474) polyclonal antibody

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Phosphoinosotode Signaling, PI3K AKT signaling, Immune Cell Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, T cell receptor signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

P-AKT2 (S474) polyclonal antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474.This antibody does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.

Western blot (WB) analysis of p-AKT2 (S474) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,15 minutes) whole cell lysate (40ug) Lane3:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:The Brain tissue lysate of Mouse (40ug) Lane6:C6 whole cell lysate (40ug) Lane7:AML-12 whole cell lysate (40ug)