AKT2 (phospho-S474) polyclonal antibody
Product Specifications
Background
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ
Swiss Prot
P31751
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, PI3K AKT signaling, Immune Cell Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, T cell receptor signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-AKT2 (S474) polyclonal antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474.This antibody does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.
Applications Notes
Western blot (WB) analysis of p-AKT2 (S474) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,15 minutes) whole cell lysate (40ug) Lane3:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:The Brain tissue lysate of Mouse (40ug) Lane6:C6 whole cell lysate (40ug) Lane7:AML-12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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