Cofilin (phospho-S3) polyclonal antibody

Product Specifications

Background

Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform; CFL1; CFL

Swiss Prot

P23528

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, Regulation of Actin Dynamics, Tumor Angiogenesis, Regulation of Microtubule Dynamics, B cell receptor signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 19 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Cofilin (S3) polyclonal antibody detects endogenous levels of Cofilin protein only when phosphorylated at Ser3.

Applications Notes

Western blot (WB) analysis of p-Cofilin (S3) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug)