Ephrin-B1 (phospho-Y317) polyclonal antibody

Product Specifications

Background

Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ephrin B1; EphrinB1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB1; EFL3; EPLG2; LERK2

Swiss Prot

P98172

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Ephrin-B1 around the phosphorylation site of Tyrosine 317.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 38, 45 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Ephrin-B1 (Y317) polyclonal antibody detects endogenous levels of Ephrin-B1 protein only when phosphorylated at Tyr317.

Applications Notes

Western blot (WB) analysis of p-Ephrin-B1 (Y317) polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate Lane2:Mouse heart tissue lysate Lane3:Rat skeletal muscle tissue lysate