C-Src (phospho-Y418) polyclonal antibody

Product Specifications

Background

The major translational products of each of the eight members of the Src gene family identified to date are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences, thereby distinguishing this group from the receptor class of tyrosine kinases. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr. The major Src gene encoded protein, c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Proto-oncogene tyrosine-protein kinase Src; 2.7.10.2; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC1; SRC

Swiss Prot

P12931

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human c-Src around the phosphorylation site of Tyrosine 418. (removal of the initiator methionine)

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Tumor Angiogenesis, Regulation of Microtubule Dynamics, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-c-Src (Y418) polyclonal antibody detects endogenous levels of c-Src protein when phosphorylated at Tyr418. (removal of the initiator methionine)

Applications Notes

Western blot (WB) analysis of p-SRC (Y418) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF (0.1ng/ML,30mins) Lane3:PC12 cell lysate treated with EGF (0.1ng/ML,30mins)