C-Src (phospho-S75) polyclonal antibody
Product Specifications
Background
The major translational products of each of the eight members of the Src gene family identified to date are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences, thereby distinguishing this group from the receptor class of tyrosine kinases. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr. The major Src gene encoded protein, c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Proto-oncogene tyrosine-protein kinase Src; 2.7.10.2; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC1; SRC
Swiss Prot
P12931
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human c-Src around the phosphorylation site of Serine 75.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, Tumor Angiogenesis, Regulation of Microtubule Dynamics, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Src (S75) polyclonal antibody detects endogenous levels of Src protein when phosphorylated at Ser75.
Applications Notes
Western blot (WB) analysis of p-c-Src (S75) pAb at 1:500 dilution Lane1:Beas-2B whole cell lysate (40µg) Lane2:HEK293T whole cell lysate (40µg) Lane3:SK-OVCAR3 whole cell lysate (40µg) Lane4:A549 whole cell lysate (40µg) Lane5:MCF-7 whole cell lysate (40µg) Lane6:H9C2 whole cell lysate (40µg)
Host or Source
Rabbit
Available Sizes
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