Csk (phospho-S364) polyclonal antibody
Product Specifications
Background
C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; CSK
Swiss Prot
P41240
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Csk around the phosphorylation site of Serine 364.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Csk (phospho-S364) polyclonal antibody detects endogenous levels of Csk protein when phosphorylated at Ser364.
Applications Notes
Western blot (WB) analysis of Csk (phospho-S364) polyclonal antibody at 1:500 dilution Lane1:CT-26 whole cell lysate (40µg) Lane2:C6 whole cell lysate (40µg) Lane3:MCF-7 whole cell lysate (40µg) Lane4:Hela whole cell lysate (40µg)
Host or Source
Rabbit
Available Sizes
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