NPM (phospho-T234) polyclonal antibody
Product Specifications
Background
Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM
Swiss Prot
P06748
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 234.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-NPM (T234) polyclonal antibody detects endogenous levels of NPM protein only when phosphorylated at Thr234.
Applications Notes
Western blot (WB) analysis of p-NPM1 (T234) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with colchicine (0.2µg/ML,24h) Lane2:Raw264.7 cell treated with colchicine (0.2µg/ML,24h) Lane3:PC12 cell lysate treated with colchicine (0.2µg/ML,24h)
Host or Source
Rabbit
Available Sizes
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