AR-β2 (phospho-S346) polyclonal antibody

Product Specifications

Background

Beta 2 Adrenergic Receptor is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca (V) 1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene contains no introns in either its coding or untranslated sequences. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. Expression of the beta 2 Adrenergic Receptor has been reported in adipose, blood, brain, heart, lung, nose, pancreas, skeletal muscle, skin, and vessel.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ADRB2; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; Beta-2 adrenergic receptor; ARβ2

Swiss Prot

P07550

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human AR-β2 around the phosphorylation site of Serine 346.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 47 kDa, 68 kDa (glycosylated), 95 kDa (Dimer)

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-AR-β2 (S346) polyclonal antibody detects endogenous levels of AR-β2 protein only when phosphorylated at Ser346.

Applications Notes

Western blot (WB) analysis of p-AR-β2 (S346) polyclonal antibody at 1:500 dilution Lane1:HepG2 cell lysate treated with PMA (100nM,30mins) Lane2:Mouse spleen tissue lysate Lane3:Rat spleen tissue lysate