APC (phospho-S2054) polyclonal antibody

Product Specifications

Background

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5; APC; DP2.5

Swiss Prot

P25054

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human APC around the phosphorylation site of Serine 2054.

Conjugation

Unconjugated

Related Pathways

Regulation of Microtubule Dynamics, Contribution of Soluble Factors to EMT

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 310 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-APC (S2054) polyclonal antibody detects endogenous levels of APC protein when phosphorylated at Ser2054.

Applications Notes

Western blot (WB) analysis of p-APC (S2054) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate