Artemis (phospho-S516) polyclonal antibody
Product Specifications
Background
Distinct DNA repair pathways minimize the consequences of mutagenic events. Reactive oxygen species (ROS) are highly reactive atoms with an unpaired electron that are conducive to double-strand DNA breaking events. Artemis, named after the Greek goddess for the protection of children, is one of the major proteins contributing to the preservation of double-strand breaks in DNA by cutting away the damaged parts of the DNA, which allows the strands to rejoin. Artemis is a single-strand-specific 5' to 3' exonuclease that forms a complex with the 469 kDa DNA-dependent protein kinase (DNA-PKcs) . DNA-PKcs phosphorylates Artemis, and Artemis acquires endonucleolytic activity on 5' and 3' overhangs and hairpins. These activities are essential for V (D) J recombination and for the 5' and 3' overhang processing in nonhomologous DNA end joining.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Protein artemis; DNA cross-link repair 1C protein; Protein A-SCID; SNM1 homolog C; hSNM1C; SNM1-like protein; DCLRE1C; ARTEMIS; ASCID; SCIDA; SNM1C
Swiss Prot
Q96SD1
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Artemis around the phosphorylation site of Serine 516.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 78 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Artemis (S516) polyclonal antibody detects endogenous levels of Protein artemis only when phosphorylated at Ser516.
Applications Notes
Western blot (WB) analysis of p-Artemis (S516) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Mouse skeletal muscle tissue lysate Lane3:Rat skeletal muscle tissue lysate
Host or Source
Rabbit
Available Sizes
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