TIP60 (phospho-S86) polyclonal antibody

Product Specifications

Background

The MOZ gene was initially isolated as a consequence of two variant translocations that were identified in a distinct subtype of acute myeloid leukemias and resulted in the formation of MOZ fusion proteins. These fusions involve the HAT domain of MOZ with the activation domain of either transcriptional co-activator protein TIF2/GRIP1 or CBP, and lead to enhanced transcriptional activation by a mechanism involving aberrant histone acetylation. Additional MOZ-related proteins, including MORF (MOZrelated factor) and TIP60 (TAT-interacting proteins 60), share significant similarities with MOZ including the putuative HAT domain. TIP60 was originally identified as a co-activator for the HIV TAT protein and also functions as a nuclear hormone receptor co-activator that enhances ligand dependent steroid receptor-mediated transactivation involving the androgen, estrogen and progesterone receptors.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA (2) -interacting protein; KAT5; HTATIP; TIP60

Swiss Prot

Q92993

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human TIP60 around the phosphorylation site of Serine 86

Conjugation

Unconjugated

Related Pathways

Examples of Crosstalk Between Post-translational Modifications, Epigenetic Writers and Erasers of Histones H2A H2B and H4

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TIP60 (phospho-S86) polyclonal antibody detects endogenous levels of TIP60 protein only when phosphorylated at Ser86.

Applications Notes

Western blot (WB) analysis of TIP60 (phospho-S86) polyclonal antibody at 1:500 dilution Lane1:The Kidney tissue lysate of Rat (40µg) Lane2:A549 whole cell lysate (40µg) Lane3:HCT116 whole cell lysate (40µg) Lane4:CT-26 whole cell lysate (40µg)