What is the catalog number for NOS3 (phospho-T494) polyclonal antibody?

The catalog number for NOS3 (phospho-T494) polyclonal antibody is BS4636.

What sizes are available for NOS3 (phospho-T494) polyclonal antibody?

NOS3 (phospho-T494) polyclonal antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does NOS3 (phospho-T494) polyclonal antibody come in?

NOS3 (phospho-T494) polyclonal antibody is available in 2 different pack sizes.

Is NOS3 (phospho-T494) polyclonal antibody in stock?

NOS3 (phospho-T494) polyclonal antibody is currently made to order / not held in immediate stock. Contact us for lead time.

NOS3 (phospho-T494) polyclonal antibody

Product Specifications

Background

NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS) ; inducible NOS (iNOS) or macrophage NOS (mNOS) ; and endothelial NOS (eNOS) .eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3

Swiss Prot

P29474

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human NOS3 around the phosphorylation site of Threonine 494.

Conjugation

Unconjugated

Related Pathways

Hypoxia Signaling, Tumor Angiogenesis

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 140 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-NOS3 (T494) polyclonal antibody detects endogenous levels of NOS3 protein when phosphorylated at Thr494. (removal of the initiator methionine)

Applications Notes

Western blot (WB) analysis of p-NOS3 (T494) pAb at 1:1000 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:SGC7901 treated with H2O2 (100nM/ml,5 minutes) whole cell lysate (40ug) Lane3:SGC7901 treated with H2O2 (100nM/ml,10 minutes) whole cell lysate (40ug)