P38 (phospho-T180) polyclonal antibody

Product Specifications

Background

MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a; MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A

Swiss Prot

Q16539

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human p38 around the phosphorylation site of Threonine 180.

Conjugation

Unconjugated

Related Pathways

Phospholipase Signaling, Immune Cell Signaling, B cell receptor signaling, MAPK Erk in Growth and Differentiation Pathway

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 42 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-p38 (T180) polyclonal antibody detects endogenous levels of p38 protein only when phosphorylated at Thr180

Applications Notes

Western blot (WB) analysis of p-p38 (T180) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with UV (4h) Lane2:Raw264.7 cell lysate treated with UV (4h) Lane3:PC12 cell lysate treated with UV (4h)