PKC δ (phospho-S645) polyclonal antibody

Product Specifications

Background

Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Sphingosine-dependent protein kinase-1; SDK1; PRKCD; PKCδ

Swiss Prot

Q05655

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Serine 645.

Conjugation

Unconjugated

Related Pathways

Protein Kinase C Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-PKC δ (S645) polyclonal antibody detects endogenous levels of PKC δ protein only when phosphorylated at Ser645.

Applications Notes

Western blot (WB) analysis of p-PKC δ (S645) pAb at 1:500 dilution Lane1:A549 whole cell lysate (20ug) Lane2:HCT116 whole cell lysate (20ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:The Uterus tissue lysate of Mouse (40ug) Lane5:The Uterus tissue lysate of Rat (40ug)