TPH1 (phospho-S260) polyclonal antibody
Product Specifications
Background
Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH
Swiss Prot
P17752
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human TPH1 around the phosphorylation site of Ser260.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 51 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TPH1 (phospho-S260) polyclonal antibody detects endogenous levels of TPH1 protein when phosphorylated at Ser260.
Applications Notes
Western blot (WB) analysis of p-TPH1 (S260) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40µg) Lane2:The Lung tissue lysate of Mouse (40µg) Lane3:LO2 whole cell lysate (40µg) Lane4:C6 whole cell lysate (40µg)
Host or Source
Rabbit
Available Sizes
Curated Selection
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