TPH1 (phospho-S260) polyclonal antibody

Product Specifications

Background

Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH

Swiss Prot

P17752

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human TPH1 around the phosphorylation site of Ser260.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 51 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TPH1 (phospho-S260) polyclonal antibody detects endogenous levels of TPH1 protein when phosphorylated at Ser260.

Applications Notes

Western blot (WB) analysis of p-TPH1 (S260) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40µg) Lane2:The Lung tissue lysate of Mouse (40µg) Lane3:LO2 whole cell lysate (40µg) Lane4:C6 whole cell lysate (40µg)