JAK2 (phospho-Y570) polyclonal antibody

Product Specifications

Background

Jak2 (Janus Kinase 2) belongs to the emerging family of non-receptor janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated Jak2. Jak2 then phosphorylates proximal STAT factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical Jak-STAT pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering Jak-STAT signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

JAK 2; JAK-2; JAK2; Janus Activating Kinase 2; Janus Kinase 2; Janus Kinase 2; JTK 10; JTK10

Swiss Prot

O60674

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human JAK2 around the phosphorylation site of Tyrosine 570.

Conjugation

Unconjugated

Related Pathways

Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 125 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-JAK2 (Y570) polyclonal antibody detects endogenous levels of JAK2 only when phosphorylated at Tyr570.

Applications Notes

Western blot (WB) analysis of p-JAK2 (Y570) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:NIH-3T3 cell lysate Lane3:Rat kidney tissue lysate