PI3K p85α/p55γ (phospho-Y467/Y199) polyclonal antibody

Product Specifications

Background

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha) . p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK; PIK3R3

Swiss Prot

P27986/Q92569

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human PI3K p85α/p55γ around the phosphorylation site of Tyrosine 467/199.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, Protein Kinase C Signaling, Regulation of Actin Dynamics, Warburg Effect, Regulation of Microtubule Dynamics, SAPK JNK Signaling Cascades, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 55, 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PI3K p85α/p55γ (phospho-Y467/Y199) polyclonal antibody detects endogenous levels of PI3K p85α only when phosphorylated at Tyr467. This antibody also detects PI3K p55γ when phosphorylated at Tyr199.

Applications Notes

Western blot (WB) analysis of PI3K p85α/p55γ (phospho-Y467/Y199) polyclonal antibody at 1:500 dilution Lane1:COS-7 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:MG63 whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:MEF whole cell lysate (40ug) Lane6:The Heart tissue lysate of Rat (40ug)