MTOR (phospho-T2446) polyclonal antibody
Product Specifications
Background
MTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Centrosomal protein of 70 kDa; Cep70; p10-binding protein; CEP70; BITE
Swiss Prot
P42345
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 246-306 of Human CEP70.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, PI3K AKT signaling, Hypoxia Signaling, Warburg Effect, Insulin &Glucose Signaling, Glutamine Metabolism, MTOR signaling, ErbB HER Signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 289 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-mTOR (T2446) pAb detects endogenous levels of mTOR protein only when phosphorylated at Thr2446.
Applications Notes
Western blot (WB) analysis of p-mTOR (T2446) pAb at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40µg) Lane2:HEK293T whole cell lysate (40µg) Lane3:CT-26 whole cell lysate (40µg) Lane4:C6 whole cell lysate (40µg)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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