MTOR (phospho-T2446) polyclonal antibody

MTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Phosphoinosotode Signaling, PI3K AKT signaling, Hypoxia Signaling, Warburg Effect, Insulin &Glucose Signaling, Glutamine Metabolism, MTOR signaling, ErbB HER Signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of p-mTOR (T2446) pAb at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40µg) Lane2:HEK293T whole cell lysate (40µg) Lane3:CT-26 whole cell lysate (40µg) Lane4:C6 whole cell lysate (40µg)